Porins of Vibrio cholerae: purification and characterization of OmpU
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چکیده
منابع مشابه
Characterization of outer membrane protein OmpU of Vibrio cholerae O1.
The outer membrane protein OmpU of Vibrio cholerae O1 strain 86B3 was characterized with reference to colonization of the intestine by the organism. The purified OmpU exhibited a pI of 3.6. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it migrated to 38, 32, and 110 kDa when the sample was heated at 100 degrees C for 2 min, 50 degrees C for 15 min, and room temperature for 30 ...
متن کاملCharacterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence.
ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viability and virulence factor expression, in-frame chromosomal deletions were constr...
متن کاملVibrio cholerae OmpU and OmpT porins are differentially affected by bile.
OmpT and OmpU are pore-forming proteins of the outer membrane of Vibrio cholerae, a pathogen that colonizes the intestine and produces cholera. Expression of the ompU and ompT genes is under the regulation of ToxR, a transmembrane transcriptional activator that also controls expression of virulence factors. It was recently shown that bile stimulates the ToxR-mediated transcription of ompU and t...
متن کاملPhenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU.
General-diffusion porins form large beta-barrel channels that control the permeability of the outer membrane of gram-negative bacteria to nutrients, some antibiotics, and external signals. Here, we have analyzed the effects of mutations in the OmpU porin of Vibrio cholerae at conserved residues that are known to affect pore properties in the Escherichia coli porins OmpF and OmpC. Various phenot...
متن کاملCharacterization of Vibrio cholerae aerotaxis.
The ability to move toward favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular, aerotaxis has been extensively studied in Escherichia coli and was shown to be dependent on the aer and tsr genes. Three putative aer gene homologs were identified in the Vibrio cholerae genome, designated aer-1 (VC0512), aer-2 (VCA0658), and aer-3 (VCA0988). Deletio...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1996
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.178.2.524-530.1996